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Macrogen sanger dna sequencing
Sanger Dna Sequencing, supplied by Macrogen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sanger+dna+sequencing/sanger+sequencing/10__1002_slash_bit__70197-47-12-15
Average 86 stars, based on 1 article reviews
sanger dna sequencing - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea
Article Snippet: .. The PCR products from positive tissues were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and amplicons were completely sequenced by Sanger DNA sequencing (Macrogen Inc., Madrid, Spain). ..

Article Title: Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in <i>Escherichia coli</i>
Article Snippet: Primers were designed using the TaKaRa Bio Primer Design tool. .. Mutant plasmids were obtained via PCR with mutagenic primers and confirmed by Sanger DNA sequencing (Macrogen, Seoul, Korea). ..

Article Title: Development and implementation of droplet digital PCR assays for accurate quantification of Plasmodium vivax parasitemia and G6PD viangchan genotyping.
Article Snippet: The reactions were performed in a 20 μL volume containing 1 × PCR buffer, 1 mM MgCl2, 1 U Taq DNA polymerase (Vivantis, Malaysia), and 0.2 mM dNTPs (Biotechrabbit, Germany). .. The PCR products were purified using purification kits (Bio-Helix, Taiwan) and sent to Macrogen (South Korea) for Sanger DNA sequencing. ..

Article Title: Epidemiological Profile and rpoB Gene Mutations in Pulmonary Tuberculosis Patients From Sulaymaniyah, Iraq: Evidence of Novel Genetic Variations and Beijing Lineage Emergence
Article Snippet: .. The PCR products were sequenced using Sanger DNA sequencing (Macrogen, South Korea), and the resulting DNA sequences were analyzed using Chromas software, Version 2.2.6. ..

Purification:

Article Title: Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea
Article Snippet: .. The PCR products from positive tissues were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and amplicons were completely sequenced by Sanger DNA sequencing (Macrogen Inc., Madrid, Spain). ..

Article Title: Development and implementation of droplet digital PCR assays for accurate quantification of Plasmodium vivax parasitemia and G6PD viangchan genotyping.
Article Snippet: The reactions were performed in a 20 μL volume containing 1 × PCR buffer, 1 mM MgCl2, 1 U Taq DNA polymerase (Vivantis, Malaysia), and 0.2 mM dNTPs (Biotechrabbit, Germany). .. The PCR products were purified using purification kits (Bio-Helix, Taiwan) and sent to Macrogen (South Korea) for Sanger DNA sequencing. ..

DNA Sequencing:

Article Title: Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of Brucella ceti in Stranded Dolphins of the Western Mediterranean Sea
Article Snippet: .. The PCR products from positive tissues were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany), and amplicons were completely sequenced by Sanger DNA sequencing (Macrogen Inc., Madrid, Spain). ..

Article Title: Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in <i>Escherichia coli</i>
Article Snippet: Primers were designed using the TaKaRa Bio Primer Design tool. .. Mutant plasmids were obtained via PCR with mutagenic primers and confirmed by Sanger DNA sequencing (Macrogen, Seoul, Korea). ..

Article Title: Development and implementation of droplet digital PCR assays for accurate quantification of Plasmodium vivax parasitemia and G6PD viangchan genotyping.
Article Snippet: The reactions were performed in a 20 μL volume containing 1 × PCR buffer, 1 mM MgCl2, 1 U Taq DNA polymerase (Vivantis, Malaysia), and 0.2 mM dNTPs (Biotechrabbit, Germany). .. The PCR products were purified using purification kits (Bio-Helix, Taiwan) and sent to Macrogen (South Korea) for Sanger DNA sequencing. ..

Article Title: Molecular identification and characterization of Wolbachia and Cardinium with co-occurrence of Leishmania spp. in Culicoides biting midges (Diptera: Ceratopogonidae) from leishmaniasis-affected areas of Thailand
Article Snippet: .. The extracted DNA was then sent to a commercial service at Macrogen Inc., South Korea, for Sanger DNA sequencing. .. All nucleotide sequences were subjected to manual editing and trimming prior to alignment using the Clustal W multiple alignment function in BioEdit sequence alignment editor v.7.2.5 ( ).

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described . .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Article Title: Epidemiological Profile and rpoB Gene Mutations in Pulmonary Tuberculosis Patients From Sulaymaniyah, Iraq: Evidence of Novel Genetic Variations and Beijing Lineage Emergence
Article Snippet: .. The PCR products were sequenced using Sanger DNA sequencing (Macrogen, South Korea), and the resulting DNA sequences were analyzed using Chromas software, Version 2.2.6. ..

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection.
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described24. .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Article Title: CRISPR/Cas9-Based Modeling of JAK2 V617F Mutation in K562 Cells Reveals Enhanced Proliferation and Sensitivity to Therapeutic Agents.
Article Snippet: The results were determined using a QX200TM Droplet Reader and QuantaSoftTM Software v.1.6 (Bio Rad Laboratories, Hercules, CA, USA). .. The positive JAK2 V617F-mutated cells identified using DDPCR were confirmed to have the JAK2 gene mutations using Sanger DNA sequencing and analysis via capillary electrophoresis at Macrogen Inc., Seoul, South Korea. ..

Mutagenesis:

Article Title: Engineering of CoA‐Acylating Butyraldehyde Dehydrogenase for Enhanced 1,3‐Butanediol Production in <i>Escherichia coli</i>
Article Snippet: Primers were designed using the TaKaRa Bio Primer Design tool. .. Mutant plasmids were obtained via PCR with mutagenic primers and confirmed by Sanger DNA sequencing (Macrogen, Seoul, Korea). ..

Virus:

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described . .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection.
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described24. .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Plaque Assay:

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described . .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Article Title: Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection.
Article Snippet: .. All virus stocks were confirmed by commercial Sanger DNA sequencing (Macrogen, Seoul, Korea) and quantitated for viral titer by plaque assay exactly as previously described24. .. Triclosan, orlistat (Merck KGaA, Darmstadt, Germany), lapatinib (Abcam, Cambridge, UK), and the mitogen-activated protein kinase (MEK) inhibitor, U0126 (Cell Signaling Technology, Danvers, MA) were dissolved in dimethyl sulfoxide (DMSO) (Merck KGaA) to obtain final stock concentrations of 100 mM for triclosan, 50 mM for lapatinib, 10 mM for U0126, and 38.5 mM for orlistat.

Software:

Article Title: Epidemiological Profile and rpoB Gene Mutations in Pulmonary Tuberculosis Patients From Sulaymaniyah, Iraq: Evidence of Novel Genetic Variations and Beijing Lineage Emergence
Article Snippet: .. The PCR products were sequenced using Sanger DNA sequencing (Macrogen, South Korea), and the resulting DNA sequences were analyzed using Chromas software, Version 2.2.6. ..

Electrophoresis:

Article Title: CRISPR/Cas9-Based Modeling of JAK2 V617F Mutation in K562 Cells Reveals Enhanced Proliferation and Sensitivity to Therapeutic Agents.
Article Snippet: The results were determined using a QX200TM Droplet Reader and QuantaSoftTM Software v.1.6 (Bio Rad Laboratories, Hercules, CA, USA). .. The positive JAK2 V617F-mutated cells identified using DDPCR were confirmed to have the JAK2 gene mutations using Sanger DNA sequencing and analysis via capillary electrophoresis at Macrogen Inc., Seoul, South Korea. ..



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A Schematic of the MB1 plasmid origin of replication (ORI) mechanism. The RNA primer is transcribed by RNA polymerase (RNAP) and, by default, folds into an RNA structure that is processed by RNase H to create a substrate for <t>DNA</t> polymerase I (Pol I) to initiate DNA replication. The antisense RNA is produced from an overlapping gene, which, if allowed to interact with the RNA primer, induces an alternative fold that does not initiate DNA replication. As the concentration of antisense RNA is proportional to the plasmid copy, this serves as a copy-control negative feedback loop. B Schematic of a refactored pMB1 ORI that separates the RNA primer and antisense RNA gene and introduces inactivating mutations in the P1 promoter <t>sequence.</t> C Shows sequences of mutations to inactivate the P1 promoter encoded inside the RNA primer. (Left graph) Whole-cell fluorescent characterization of the mutant promoters using a fluorescent reporter gene. (Right graph) Relative copy number of plasmids containing the original pMB1 ORI or refactored ORI with an RNA primer containing mutant P1 promoters. Copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid. Fluorescence characterization was performed (measured in units of fluorescence/optical density (OD) at 600 nm) in E. coli cells. Data show mean values ± SD and individual values of n = 4 biological replicates. Source data for this figure is available in the Source Data file.
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A Schematic of the MB1 plasmid origin of replication (ORI) mechanism. The RNA primer is transcribed by RNA polymerase (RNAP) and, by default, folds into an RNA structure that is processed by RNase H to create a substrate for <t>DNA</t> polymerase I (Pol I) to initiate DNA replication. The antisense RNA is produced from an overlapping gene, which, if allowed to interact with the RNA primer, induces an alternative fold that does not initiate DNA replication. As the concentration of antisense RNA is proportional to the plasmid copy, this serves as a copy-control negative feedback loop. B Schematic of a refactored pMB1 ORI that separates the RNA primer and antisense RNA gene and introduces inactivating mutations in the P1 promoter <t>sequence.</t> C Shows sequences of mutations to inactivate the P1 promoter encoded inside the RNA primer. (Left graph) Whole-cell fluorescent characterization of the mutant promoters using a fluorescent reporter gene. (Right graph) Relative copy number of plasmids containing the original pMB1 ORI or refactored ORI with an RNA primer containing mutant P1 promoters. Copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid. Fluorescence characterization was performed (measured in units of fluorescence/optical density (OD) at 600 nm) in E. coli cells. Data show mean values ± SD and individual values of n = 4 biological replicates. Source data for this figure is available in the Source Data file.
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A Schematic of the MB1 plasmid origin of replication (ORI) mechanism. The RNA primer is transcribed by RNA polymerase (RNAP) and, by default, folds into an RNA structure that is processed by RNase H to create a substrate for DNA polymerase I (Pol I) to initiate DNA replication. The antisense RNA is produced from an overlapping gene, which, if allowed to interact with the RNA primer, induces an alternative fold that does not initiate DNA replication. As the concentration of antisense RNA is proportional to the plasmid copy, this serves as a copy-control negative feedback loop. B Schematic of a refactored pMB1 ORI that separates the RNA primer and antisense RNA gene and introduces inactivating mutations in the P1 promoter sequence. C Shows sequences of mutations to inactivate the P1 promoter encoded inside the RNA primer. (Left graph) Whole-cell fluorescent characterization of the mutant promoters using a fluorescent reporter gene. (Right graph) Relative copy number of plasmids containing the original pMB1 ORI or refactored ORI with an RNA primer containing mutant P1 promoters. Copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid. Fluorescence characterization was performed (measured in units of fluorescence/optical density (OD) at 600 nm) in E. coli cells. Data show mean values ± SD and individual values of n = 4 biological replicates. Source data for this figure is available in the Source Data file.

Journal: Nature Communications

Article Title: Engineering plasmids with synthetic origins of replication

doi: 10.1038/s41467-026-68907-1

Figure Lengend Snippet: A Schematic of the MB1 plasmid origin of replication (ORI) mechanism. The RNA primer is transcribed by RNA polymerase (RNAP) and, by default, folds into an RNA structure that is processed by RNase H to create a substrate for DNA polymerase I (Pol I) to initiate DNA replication. The antisense RNA is produced from an overlapping gene, which, if allowed to interact with the RNA primer, induces an alternative fold that does not initiate DNA replication. As the concentration of antisense RNA is proportional to the plasmid copy, this serves as a copy-control negative feedback loop. B Schematic of a refactored pMB1 ORI that separates the RNA primer and antisense RNA gene and introduces inactivating mutations in the P1 promoter sequence. C Shows sequences of mutations to inactivate the P1 promoter encoded inside the RNA primer. (Left graph) Whole-cell fluorescent characterization of the mutant promoters using a fluorescent reporter gene. (Right graph) Relative copy number of plasmids containing the original pMB1 ORI or refactored ORI with an RNA primer containing mutant P1 promoters. Copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid. Fluorescence characterization was performed (measured in units of fluorescence/optical density (OD) at 600 nm) in E. coli cells. Data show mean values ± SD and individual values of n = 4 biological replicates. Source data for this figure is available in the Source Data file.

Article Snippet: All assembled plasmids were verified using Sanger DNA sequencing (Genewiz) or Nanopore sequencing (Plasmidsaurus).

Techniques: Plasmid Preparation, Produced, Concentration Assay, Control, Sequencing, Mutagenesis, Expressing, Fluorescence

A SynORI plasmids (pSynORI) can be programmed to convert distinct chemical signals into DNA copy output using transcriptional regulators to control RNA primer transcription. Relationship between relative copy number and chemical input for pSynORI built using (B) Plac (activated by IPTG), C PcymR (activated by cumate), D the ZTP riboswitch (activated by Z), E and the yxjA riboswitch (repressed by 2AP). F Matrix showing the relative copy of a pSynORI that is activated by cumate and repressed by IPTG with inducer titrations. Relative copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid in E. coli cells (measured in units of fluorescence/optical density [OD] at 600 nm). G Schematic of compatible and inducible pSynORI that converts chemical input signals into a DNA copy, which can be read using sequencing. A pSC101 plasmid with a non-inducible copy number is included as a reference. H The relative copy of pSynORI A and B was calculated using the reference plasmid. Bar plots show the DNA copy ratio of pSynORI A and B. Data in B – E and F shows n = 4 biological replicates, and Data in H show n = 3 biological replicates. All error bands indicate SD. Source data for this figure is available in the Source Data file.

Journal: Nature Communications

Article Title: Engineering plasmids with synthetic origins of replication

doi: 10.1038/s41467-026-68907-1

Figure Lengend Snippet: A SynORI plasmids (pSynORI) can be programmed to convert distinct chemical signals into DNA copy output using transcriptional regulators to control RNA primer transcription. Relationship between relative copy number and chemical input for pSynORI built using (B) Plac (activated by IPTG), C PcymR (activated by cumate), D the ZTP riboswitch (activated by Z), E and the yxjA riboswitch (repressed by 2AP). F Matrix showing the relative copy of a pSynORI that is activated by cumate and repressed by IPTG with inducer titrations. Relative copy number was characterized by encoding a constitutive RFP expression cassette onto the plasmid in E. coli cells (measured in units of fluorescence/optical density [OD] at 600 nm). G Schematic of compatible and inducible pSynORI that converts chemical input signals into a DNA copy, which can be read using sequencing. A pSC101 plasmid with a non-inducible copy number is included as a reference. H The relative copy of pSynORI A and B was calculated using the reference plasmid. Bar plots show the DNA copy ratio of pSynORI A and B. Data in B – E and F shows n = 4 biological replicates, and Data in H show n = 3 biological replicates. All error bands indicate SD. Source data for this figure is available in the Source Data file.

Article Snippet: All assembled plasmids were verified using Sanger DNA sequencing (Genewiz) or Nanopore sequencing (Plasmidsaurus).

Techniques: Control, Expressing, Plasmid Preparation, Fluorescence, Sequencing